Royal L #39;Opulent Rejuv
Rejuvenate your skin through the activation of skin stem cells. Brightens your skin for a more even toned complexion, restores skins natural moisture to serv...

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Royal L'Opulent Rejuv - Video

The advance could lead to tissue-transplant operations for a range of debilitating disorders, such as Parkinson's disease, multiple sclerosis, heart disease and spinal cord injuries.

Last year, a team created stem cells from the skin cells of babies, but it was unclear whether it would work in adults.

However, a team of scientists from the Research Institute for Stem Cell Research at CHA Health Systems in Los Angeles and the University of Seoul said they had achieved the same result with two men, one aged 35, the other the 75-year-old. "The proportion of diseases you can treat with lab-made tissue increases with age. So if you can't do this with adult cells it is of limited value," said Robert Lanza, co-author of the research, which was published in the journal Cell Stem Cell.

The technique works by removing the nucleus from an unfertilised egg and replacing it with the nucleus of a skin cell. An electric shock causes the cells to divide until they form a "blastocyst", a small ball of a few hundred cells.

In IVF, a blastocyst is implanted into the womb, but with the new technique the cells would be harvested to create other organs or tissues.

The breakthrough is likely to reignite the debate about the ethics of creating human embryos for medical purposes and the possible use of the same technique to produce cloned babies - which is illegal in Britain.

Although the embryos created may not produce a human clone even if implanted in a womb, the prospect is now closer. However, scientists have tried for years to clone monkeys and have yet to succeed.

Dr Lanza admitted that without strong regulations, the early embryos produced in therapeutic cloning "could also be used for human reproductive cloning, although this would be unsafe and grossly unethical". However, he said it was important for the future of regenerative medicine that research into therapeutic cloning should continue.

Shoukhrat Mitalipov, a reproductive biologist from Oregon Health and Science University, who developed the technique last year, said: "The advance here is showing that [nuclear transfer] looks like it will work with people of all ages.

"I'm happy to hear that our experiment was verified and shown to be genuine."

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Cloning advance means human tissues could be regrown, even in old age

Ever since Dolly the Sheep was cloned in 1996, scientists have been trying to do the same thing with human cells. Using the same technique, scientists say they've finally accomplished the feat with adult cells.

"What we show for the first time is that you can actually take skin cells, from a middle-aged 35-year-old male, but also from an elderly, 75-year-old male" and use the DNA to create tissue with cells of an exact match, said co-author of the study Robert Lanza.

The work was published in the journal Cell Stem Cell.

Last year, the technique was successfully used with infant cells, but in order to create tissue in a lab that could treat adult diseases, such as Alzheimer's, scientists needed to know if the technique would work with adult cells.

"I'm happy to hear that our experiment was verified and shown to be genuine," said Shoukhrat Mitalipov, a development biologist at Oregon Health and Science University, who led the 2013 study.

The work confirmed that starting with a quality human egg is key to the process. The researchers replaced the original DNA in an unfertilized egg with the donor DNA, and then cultured the cells in a lab dish. The stem cells, which were an exact match to the donor's DNA, can then be turned into various tissue types.

Even though full human cloning is a long way off, the report may raise an equal amount of concern and excitement.

"Certainly this kind of technology could be abused by some kind of rogue scientist," Paul Knoepfler of the University of California, Davis, School of Medicine, told NPR.

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Adult Human Cells Cloned for First Time

A breakthrough in human stem cell research could lead to the treatment of countless diseases, invaluable scientific research and yes, human cloning.

According to a study in the journalCell Stem Cell, scientists have synthesized human embryonic stem cells from the cells of adults, creating two different lines from the skin of two donors.

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Using the nuclear transfermethod,scientists took DNA out of egg cells and replaced it with the donor DNA. The cells were basically reprogrammed, butof the 77 samplesonly two fully developed into cloned stem cells.

Lead researcher Robert Lanza says the 5 percent success rate isn't surprising."Reprogramming is more difficult for adult cells than for fetal [and] infant cells, presumably at least in part because their epigenetic landscape from the pluripotent state,"meaning the cells generally dont' have the right enzymes for change anymore.

The researchers reportedly tweaked a method made famous by the cloning of the sheep Dolly in 1996 and improved by scientists at Oregon Health & Science University just last year.

The nuclear transfermethod is the third discovered way to harvest or create stem cells. In the past, scientists have extracted cells from leftover embryos after in vitro fertilizations,a controversial practice. And in 2006 aJapanese researcher discovered a way to create themby injecting new genes. (ViaAsian Scientist)

Lanza's method could provide easy access to stem cells, opening up new research intodiseases like diabetes, Parkinsons and even leukemia. And according toNPR, the researcher wants to create a virtual library of cells using carefully selected DNA donors.

The implications of a real and viable approach for creating stem cells could be startling, andscientists have been wrestling with the ethical questions since the cloning of Dolly.

An official at Oregon Health & Science Universitythinks studying stemcells is necessary, tellingTime,They have become kind of like cursed cells. But we clearly need to understand more about them.

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Scientists create stem cells from adult skin cells

Scientists have replicated one of the most significant accomplishments in stem cell research by creating human embryos that were clones of two men.

The lab-engineered embryos were harvested within days and used to create lines of infinitely reproducing embryonic stem cells, which are capable of growing into any type of human tissue.

The work, reported Thursday in the journal Cell Stem Cell, comes 11 months after researchers in Oregon said they had produced the world's first human embryo clones and used them to make stem cells. Their study, published in Cell, aroused skepticism after critics pointed out multiple errors and duplicated images.

In addition, the entire effort to clone human embryos and then dismantle them in the name of science troubles some people on moral grounds.

MORE: Medicines and machines, inspired by nature

The scientists in Oregon and the authors of the new report acknowledged that the clones they created could develop into babies if implanted in surrogate wombs. But like others in the field, they have said reproductive cloning would be unethical and irresponsible.

The process used to create cloned embryos is called somatic cell nuclear transfer, or SCNT. It involves removing the nucleus from an egg cell and replacing it with a nucleus from a cell of the person to be cloned. The same method was used to create Dolly the sheep in 1996, along with numerous animals from other species.

Human cloning was a particular challenge, in part because scientists had trouble getting enough donor eggs to carry out their experiments. Some scientists said SCNT in humans would be impossible.

Dr. Robert Lanza, the chief scientific officer for Advanced Cell Technology Inc. in Marlborough, Mass., has been working on SCNT off and on for about 15 years. He and his colleagues finally achieved success with a modified version of the recipe used by the Oregon team and skin cells donated by two men who were 35 and 75.

After swapping out the nucleus in the egg cell, both groups used caffeine to delay the onset of cell division a technique that has been called "the Starbucks effect." But instead of waiting 30 minutes to prompt cell division, as was done in the Oregon experiment, Lanza and his team waited two hours.

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Scientists use cloning to make stem cells matched to two adults

April 18, 2014

Brett Smith for redOrbit.com Your Universe Online

In a significant breakthrough a team of scientists from California and Seoul, South Korea have been able to create viable stem cells from an adult donor that perfectly match the donors DNA, according to a new report in the journal Cell Stem Cell.

The development, referred to as therapeutic cloning, involves the production of embryonic cells for scientific purposes and many object to this type of research based on moral or religious grounds. Debate over this type of work was stoked in 1997 with the announcement that it was used to create the clone of a sheep, called Dolly. In 2005, the United Nations called for a ban on cloning and the United States government currently prohibits the use of federal dollars for cloning research.

The scientists behind the latest development, which was partially funded by the government of South Korea, acknowledged that if the embryos in their study were implanted in a uterus they could have developed into a fetus.

Without regulations in place, such embryos could also be used for human reproductive cloning, although this would be unsafe and grossly unethical, study author Dr. Robert Lanza, chief scientist of Massachusetts-based biotech Advanced Cell Technology, told Reuters reporter Sharon Begley.

To produce viable stem cells from an adult donor, the researchers first inserted DNA from an adult skin cell into a donated ovum. The scientists then delivered an electric shock to fuse the genetic material to the ovum. Eventually, the ovum divides and multiplies becoming a viable embryo in five or six days. Pluripotent stem cells, which can become any type of cell in the body, are located on the interior of this embryo.

Last year, a team of Oregon scientists reported on their success in combining genetic material from fetal and infant cells with DNA-extracted eggs. The team was able to develop their eggs into approximately 150-cell embryos.

The Oregon team said a major aspect of their success was allowing the engineered eggs to sit for 30 minutes before hitting them with the charge of electricity that like Dr. Frankensteins monster set the eggs on the path to becoming alive.

In the new study, the researchers waited two hours before triggering the egg, which Lanza said allowed them to succeed.

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Stem Cells Created From Adult Cells

Scientists have moved a step closer to the goal of creating stem cells perfectly matched to a patient's DNA in order to treat diseases, they announced on Thursday, creating patient-specific cell lines out of the skin cells of two adult men.

The advance, described online in the journal Cell Stem Cell, is the first time researchers have achieved "therapeutic cloning" of adults. Technically called somatic-cell nuclear transfer, therapeutic cloning means producing embryonic cells genetically identical to a donor, usually for the purpose of using those cells to treat disease.

But nuclear transfer is also the first step in reproductive cloning, or producing a genetic duplicate of someone - a technique that has sparked controversy since the 1997 announcement that it was used to create Dolly, the clone of a ewe. In 2005, the United Nations called on countries to ban it, and the United States prohibits the use of federal funds for either reproductive or therapeutic cloning.

The new study was funded by a foundation and the South Korean government.

If confirmed by other labs, it could prove significant because many illnesses that might one day be treated with stem cells, such as heart failure and vision loss, primarily affect adults. Patient-specific stem cells would have to be created from older cells, not infant or fetal ones. That now looks possible, though far from easy: Out of 39 tries, the scientists created stem cells only once for each donor.

Outside experts had different views of the study, which was led by Young Gie Chung of the Research Institute for Stem Cell Research at CHA Health Systems in Los Angeles.

Stem cell biologist George Daley of the Harvard Stem Cell Institute called it "an incremental advance" and "not earth-shattering."

Reproductive biologist Shoukhrat Mitalipov of Oregon Health and Science University, who developed the technique the CHA team adapted, was more positive. "The advance here is showing that (nuclear transfer) looks like it will work with people of all ages," he said in an interview.

A year ago, Mitalipov led the team that used nuclear transfer of fetal and infant DNA to produce stem cells, the first time that had been accomplished in humans of any age.

ELECTRIC JOLT

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Scientists create stem cells from adults

hide captionThis mouse egg (top) is being injected with genetic material from an adult cell to ultimately create an embryo and, eventually, embryonic stem cells. The process has been difficult to do with human cells.

Eighteen years ago, scientists in Scotland took the nuclear DNA from the cell of an adult sheep and put it into another sheep's egg cell that had been emptied of its own nucleus. The resulting egg was implanted in the womb of a third sheep, and the result was Dolly, the first clone of a mammal.

Dolly's birth set off a huge outpouring of ethical concern along with hope that the same techniques, applied to human cells, could be used to treat myriad diseases.

But Dolly's birth also triggered years of frustration. It's proved very difficult to do that same sort of DNA transfer into a human egg.

Last year, scientists in Oregon said they'd finally done it, using DNA taken from infants. Robert Lanza, chief scientific officer at Advanced Cell Technology, says that was an important step, but not ideal for medical purposes.

"There are many diseases, whether it's diabetes, Alzheimer's or Parkinson's disease, that usually increase with age," Lanza says. So ideally scientists would like to be able to extract DNA from the cells of older people not just cells from infants to create therapies for adult diseases.

Lanza's colleagues, including Young Gie Chung at the CHA Stem Cell Institute in Seoul, Korea (with labs in Los Angeles as well), now report success.

Writing in the journal Cell Stem Cell, they say they started with nuclear DNA extracted from the skin cells of a middle-age man and injected it into human eggs donated by four women. As with Dolly, the women's nuclear DNA had been removed from these eggs before the man's DNA was injected. They repeated the process this time starting with the genetic material extracted from the skin cells of a much older man.

hide captionDolly, the first mammal to be genetically cloned from adult cells, poses for the camera in 1997 at the Roslin Institute in Edinburgh, Scotland.

Dolly, the first mammal to be genetically cloned from adult cells, poses for the camera in 1997 at the Roslin Institute in Edinburgh, Scotland.

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First Embryonic Stem Cells Cloned From A Man's Skin

Researchers say they have made powerful stem cells from both young and old adults using cloning techniques, and also found clues about why it is so difficult to do this with human beings.

The team, at Massachusetts-based Advanced Cell Technology and the Institute for Stem Cell Research in Los Angeles, say they used the cloning methods to create the stem cells to match a 35-year-old man and a 75-year-old man.

They used a bit of skin from each man, took the DNA from the skin cells and inserted it into the egg cell of a female donor, and grew very early embryos called blastocysts, the team reports in the journal Cell Stem Cell. Cells from these embryos closely match the men and could, in theory, be used to make near-identical tissue, blood or organ transplants for the men.

If verified, it would be only the second confirmed time someones been able to use cloning methods to make human embryonic stem cells, considered the bodys master cells.

Therapeutic cloning has long been envisioned as a means for generating patient-specific stem cells that could be used to treat a range of age-related diseases, said Dr. Robert Lanza, chief scientific officer for Advanced Cell Technology.

However, despite cloning success in animals, the derivation of stem cells from cloned human embryos has proven elusive. Only one group has ever succeeded, and their lines were generated using fetal and infant cells.

That was last year, at Oregon Health & Science University.

When human embryonic stem cells were first discovered in 1998, scientists immediately dreamed of using cloning technology to help people grow their own organ and tissue transplants, and to use them to study disease. Theyd be perfect genetic matches for each patient, meaning an end to a lifetime of taking dangerous immune-suppressing drugs after an organ transplant.

But in the many years since, no labs been able to do the work easily. It seems it is much harder to clone a human being than it is to clone a sheep, a frog or a mouse.

And using the cloning technique is controversial, because it involves creating, then destroying, a human embryo.

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Group Makes Stem Cells Using Clone Technique

Scientists have replicated one of the most significant accomplishments in stem cell research by creating human embryos that were clones of two men.

The lab-engineered embryos were harvested within days and used to create lines of infinitely reproducing embryonic stem cells, which are capable of growing into any type of human tissue.

The work, reported Thursday in the journal Cell Stem Cell, comes 11 months after researchers in Oregon said they had produced the world's first human embryo clones and used them to make stem cells. Their study, published in Cell, aroused skepticism after critics pointed out multiple errors and duplicated images.

In addition, the entire effort to clone human embryos and then dismantle them in the name of science troubles some people on moral grounds.

The scientists in Oregon and the authors of the new report acknowledged that the clones they created could develop into babies if implanted in surrogate wombs. But like others in the field, they have said reproductive cloning would be unethical and irresponsible.

The process used to create cloned embryos is called somatic cell nuclear transfer, or SCNT. It involves removing the nucleus from an egg cell and replacing it with a nucleus from a cell of the person to be cloned. The same method was used to create Dolly the sheep in 1996, along with numerous animals from other species.

Human cloning was a particular challenge, in part because scientists had trouble getting enough donor eggs to carry out their experiments. Some scientists said SCNT in humans would be impossible.

Dr. Robert Lanza, the chief scientific officer for Advanced Cell Technology Inc. in Marlborough, Mass., has been working on SCNT off and on for about 15 years. He and his colleagues finally achieved success with a modified version of the recipe used by the Oregon team and skin cells donated by two men who were 35 and 75.

After swapping out the nucleus in the egg cell, both groups used caffeine to delay the onset of cell division a technique that has been called "the Starbucks effect." But instead of waiting 30 minutes to prompt cell division, as was done in the Oregon experiment, Lanza and his team waited two hours.

It remains unclear exactly how the egg causes the cells in previously mature tissues in this case, skin to transform into a more versatile, pluripotent state.

More here:
Results are a leap for embryonic stem cells

However a team at the Research Institute for Stem Cell Research at CHA Health Systems in Los Angeles and the University of Seoul said they had achieved the same result with two men, one aged 35 and one 75.

"The proportion of diseases you can treat with lab-made tissue increases with age. So if you cant do this with adult cells it is of limited value, said Robert Lanza, co-author of the research which published in the journal Cell Stem Cell

The technique works by removing the nucleus from an unfertilised egg and replacing it with the nucleus of a skin cell. An electric shock causes the cells to begin dividing until they form a blastocyst a small ball of a few hundred cells.

In IVF it is a blastocyst which is implanted into the womb, but with this technique the cells would be harvested to be used to create other organs or tissues.

However, the breakthrough is likely to reignite the debate about the ethics of creating human embryos for medical purposes and the possible use of the same technique to produce cloned babies which is illegal in Britain.

Although the embryos created may not give rise to a human clone even if implanted in a womb, the prospect is now scientifically closer.

However scientists have been trying for years to clone monkeys and have yet to succeed.

Dr Lanza admitted that without strong regulations, the early embryos produced in therapeutic cloning could also be used for human reproductive cloning, although this would be unsafe and grossly unethical.

However, he said it was important for the future of regenerative medicine that research into therapeutic cloning should continue.

Reproductive biologist Shoukhrat Mitalipov of Oregon Health and Science University, who developed the technique last year said: "The advance here is showing that (nuclear transfer) looks like it will work with people of all ages.

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Breakthrough in human cloning offers new transplant hope

For the first time, scientists have created early-stage embryos using cloned cells from adults.

A study from Advanced Cell Technology published Thursday in the journal Cell Stem Cell highlights how researchers were able to create embryos from the skin cells of two men, ages 35 and 75. Tissue from the embryos featured exact DNA matches with the donors.

Last year, scientists at Oregon Health and Science University made a major breakthrough with the first early-stage human clones derived from infant and fetal cells. However, the experiment drew criticism because early-stage human embryos are destroyed when cells are extracted from them.

This more recent experiment involving adult cells, funded by the South Korean government and performed in California, has large implications for advances in medical treatment. However, the success ratio was low: Scientists attempted 39 times to create stem cells but succeeded only once with each donor.

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Scientists Clone Stem Cells From Adults For The First Time

Scientists have used steroids to enhance the performance of stem cells (Photo: Shutterstock)

Stem cells are highly promising for the treatment of everything from HIV to leukemia to baldness. In many cases, however, a great number of them must be used in order have a noticeable effect, which makes treatments impractical or expensive. Now, scientists at Harvard-affiliated Brigham and Women's Hospital have found that a smaller number of stem cells can still get the job done, if they're first hopped up on steroids.

The research was conducted by Doctors Jeffrey Karp and James Ankrum, the former of whom has also helped bring us painless medical tape for newborns, worm-inspired skin grafts, porcupine quill-inspired surgical patches, and superglue for holes in the heart.

The scientists started with ordinary mesenchymal stem cells, and treated them with glucocorticoid steroids. This caused the cells to produce an increased amount of indoleamine-2,3-dioxygenase (IDO), which is an anti-inflammatory agent. Since it was noted that the cells' IDO expression was highest when they were actually being exposed to the steroids, the scientists added steroid-containing microparticles to the cells, so that they could have access to the drugs at all times.

When the 'roided-up stem cells were then introduced to inflamed immune cells, they were found to reduce inflammation twice as effectively as unmodified mesenchymal stem cells.

"Our approach enables fine tuning of cell potency and control following transplantation, which could lead to more successful cell-based therapies," said Ankrum.

A paper on the research was recently published in the journal Scientific Reports.

Source: Brigham and Women's Hospital

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Original post:
Scientists give stem cells a performance boost ... by putting them on steroids

When researchers and, especially, the general public became aware of the potential medical uses of stem cells the possibilities seemed endless. The National Institutes of Health said this: ... a renewable source of replacement cells and tissues to treat a myriad of diseases, conditions, and disabilities, including Parkinsons disease, amyotrophic lateral sclerosis, spinal cord injury, burns, heart disease, diabetes, and arthritis.

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EDITORIAL: London researchers illustrate potential of stem cell therapies

See Inside

For more than 40 years government officials have grappled with how to regulate and fund the controversial research

Despite its promise, stem cell research in the U.S. has been stymied, time and again, by bioethical landmines. The explosive debate revolves around the fact that, until recently, the only way to get pluripotent stem cells was to extract them from human embryos left over from in-vitro fertilizationa process that destroyed the five-day-old embryo. The ongoing debate about when life begins has led many to oppose stem cell research on the grounds that it is immoral to destroy something that could eventually grow into a person. On the other hand, promoters argue that the potential to help millions of people with stem cell therapies outweighs the sanctity of cells that are not viable outside the womb and that often go unused. Arguments on both sides are based on personal beliefs that may never be reconciled, so the debate hinges on whether the federal government should fund research that many citizens find morally objectionable. The following box chronicles stem cell research regulation in the U.S.

1970s

The Supreme Court legalizes abortion in 1973. The ensuing debate on the ethics of experimenting on fetal tissue prompts Congress to issue a moratorium on federal funding for research on human embryos the following year.

1990s

In 1995 President Clinton lifts the ban on funding for study of stem cells left over from in-vitro fertilization, but leaves other restrictions in place. In response, Congress passes the Dickey-Wicker Amendment, prohibiting funding for all research in which a human embryo or embryos are destroyed, discarded, or knowingly subjected to risk of injury or death, regardless of the source of the embryo.

2000s

President George W. Bush announces that federal funding will be made available for research on the approximately 60 existing embryonic stem cell lines, but not new ones. Congress twice votes to loosen the restrictions on funding for research using embryonic stem cells left over from in-vitro fertilization but President Bush vetoes the legislation both times.

In 2009, early in his first term, President Barack Obama removes the ban on federal funding for new stem cell lines but signs an omnibus bill preserving the Dickey-Wicker Amendment. The move retains restrictions against federal funding for the direct creation of new stem cell lines, but opens up funding for research on newly created lines developed with private or state money.

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Dispute over Stem Cells: A Timeline

Carlsbad, CA (PRWEB) April 10, 2014

Most baby boomers grew up not knowing about the importance of sun protection. The term SPF wasnt even invented until 1962. So lets blame those lines and wrinkles, age spots and skin laxity on the sun! According to the Environmental Protection Agency, as much as 90 percent of skin aging is caused by sun exposure. New ingredients are emerging that are changing the long-held belief that UV skin damage is irreversible. In fact, studies show when the skin is exposed to extracts from human stem cells it helps repair and rejuvenate itself. Lifeline Skin Care, the only line of skin care products in the world based on growth factors from human stem cells, will launch its new Daily Defense Complex in April in spas and physician offices nationwide. The super-potent formula will firm, tone and defend skin and also integrates easily into post-procedure protocols and homecare regimens.

Lifeline Skin Care uses growth factors that have been extracted from human stem cells, said Simon Craw, Ph.D., of International Stem Cell Corporation, the parent company of Lifeline Skin Care. Stem cells have the natural ability to identify and repair damaged cells. In the laboratory, we discovered how stem cells can rejuvenate many different types of cells, including skin cells. The proteins and growth factors that are extracted from these stem cells can reduce the appearance of the signs of aging--lines, wrinkles and loss of radiance.

Dermatologists believe that women dont get serious about anti-aging skin care until theyre in their 30s, when fine lines and wrinkles begin to appear. But the 40th birthday is the real game changer, said Dr. Elizabeth Hale of Complete Skin MD in New York City. After age 40, fine lines deepen into full fledged wrinkles, and dark spots and age spots begin to surface. Its at this point that women start to look for more advanced and results-oriented skin care ingredients.

Key ingredients in the new Daily Defense Complex help to repair previous photoaging and protect against future UV damage. Collagen and elastin production have been proven in vitro to increase by 46-55%. Collagen and elastin are two key proteins that make skin appear firmer and younger-looking.

Daily Defense Complex is designed for all skin types but it is particularly recommended for mature or photodamaged skin. It retails for $160.00 and is available from physicians, spas and lifelineskincare.com. For more information, please visit http://www.lifelineskincare.com

About Lifeline Skin Care Lifeline Skin Care develops, markets and sells advanced topical anti-aging skin care products based on technology developed and patented by International Stem Cell Corporation. The technology uses ingredients that have been extracted from ISCOs human, parthenogenetic stem cells and are known to reduce the visible signs of skin aging. Lifeline is distributed in the USA and internationally through physicians and spas. For more information visit http://www.lifelineskincare.com

About International Stem Cell Corporation International Stem Cell Corporation is focused on the therapeutic applications of human parthenogenetic stem cells (hpSCs) and the development and commercialization of cell-based research and cosmetic products. ISCO's core technology, parthenogenesis, results in the creation of pluripotent human stem cells from unfertilized oocytes (eggs) hence avoiding ethical issues associated with the use or destruction of viable human embryos. ISCO scientists have created the first parthenogenetic, homozygous stem cell line that can be a source of therapeutic cells for hundreds of millions of individuals of differing genders, ages and racial background with minimal immune rejection after transplantation. hpSCs offer the potential to create the first true stem cell bank, UniStemCell. ISCO also produces and markets specialized cells and growth media for therapeutic research worldwide through its subsidiary Lifeline Cell Technology (http://www.lifelinecelltech.com), and stem cell-based skin care products through its subsidiary Lifeline Skin Care (http://www.lifelineskincare.com). More information is available at http://www.internationalstemcell.com.

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Lifeline Skin Care Launches Daily Defense Anti-Aging Skincare Complex Using Groundbreaking Technology and Science to ...

13 hours ago Isolated planarian pharynx: two tissue types in this digestive organ are shown. In red, cilia of the epithelial layer ensheathing the organ are labeled with an antibody against acetylated tubulin. In green, the complex longitudinal and circular muscle fibers are shown as labeled by the anti-myosin heavy chain antibody Tmus-13. Credit: Carrie Adler, Ph.D., Stowers Institute for Medical Research

As multicellular creatures go, planaria worms are hardly glamorous. To say they appear rudimentary is more like it. These tiny aquatic flatworms that troll ponds and standing water resemble brown tubes equipped with just the basics: a pair of beady light-sensing "eyespots" on their head and a feeding tube called the pharynx (which doubles as the excretory tract) that protrudes from a belly sac to suck up food. It's hard to feel kinship with them.

But admiration is another thing, because many planaria species regenerate in wondrous waysnamely, when quartered they reconstruct themselves from the pieces. Sliced through the "waist", they regenerate the missing tail or head; bisected lengthwise, worms duplicate their mirror image. This capacity is not what's surprising, as biologists know that 30% of their body cells are stem cells. The question is, how do stem cells in a planaria fragment know how to generate what's missing?

In the April 15, 2014 issue of the online journal eLife, Stowers Institute for Medical Research Investigator Alejandro Snchez Alvarado and colleagues address that issue by identifying genes worms use to rebuild an amputated pharynx. They report that near the top of the pharynx regeneration hierarchy is a master regulator called FoxA. These findings support an evolutionarily conserved role for FoxA proteins in driving construction of endoderm-derived organs and reveal how stem cells sense loss of a particular structure on a molecular level.

Mammals can deploy adult stem cells to replace skin or immune system cells, among others. But when it comes to re-creating entire structures, amphibian, fish and planarian species are the champs. "When mammals are severely injured, they just heal the wound and call it a day," says Snchez Alvarado, who is also a Howard Hughes Medical Institute Investigator. "But if a salamander loses a limb, it will first heal the wound and then start assembling the missing parts. Right now, the mechanisms cells use to realize what structure is missing and then restore it remain completely mysterious."

To unravel the mystery, the team conducted two "screens". First, they amputated the worm pharynx, which prohibits feeding for about a week as planaria rebuild a new one. Around day 3 post-amputation, the team conducted microarray analysis to identify any gene switched on by amputation and amassed about 350 candidates. To test them, they then fed inhibitory RNAs designed to suppress expression of each gene separately to new batches of worms, repeated the amputations and observed whether worms regained feeding ability. That narrowed the list to 20 candidates that when lost hampered feeding and in most cases interfered with pharynx formation.

According to Carrie Adler, Ph.D., a postdoctoral fellow in the Snchez Alvarado lab who led the study, analysis showed most of the 20 factors either had a generic function in stem cells (which was interesting but not what they were after) or were specifically required for pharynx regeneration. Among the latter, one factor showing a particularly robust effect was a DNA-binding protein called FoxA. "Targeting FoxA completely blocked pharynx regeneration but had no effect on the regeneration of other organs," says Adler.

High resolution microscopy analysis showed that stem cells ramped up FoxA expression soon after they converged on the amputation site. "Currently, we think that FoxA triggers a cascade of gene expression that drives stem cells to produce all of the different cells of the pharynx, including muscle, neurons, and epithelial cells," says Adler. "The next question is how FoxA gets stimulated in the first place in only some stem cells."

Researchers knew previously that during embryogenesis FoxA initiates formation of endoderm-derived organs in species as diverse as mouse and roundworms. The new work suggests that regenerating tissues exploit those evolutionarily ancient gene expression pathways. "Engulfing food is one thing that defines an animal," says Snchez Alvarado. "This means that organisms from humans to flatworms use a common toolbox to build a digestive system, one that has been shared since animals became multicellular."

A fortuitous (in hindsight) setback facilitated the work. As a graduate student studying the roundworm C. elegans, Adler decided to test effects of roundworm anesthetics on flatworms. One, a sodium azide bath, put planaria to sleep but made their pharynxes drop off. Aghast, Adler soon realized that the azide solution (which planaria survived) left a uniform, minimally-destructive lesion. Thus was born the "selective chemical amputation method", allowing large-scale analysis and reliable quantification of results and freeing researchers from tedious hours at a dissecting microscope.

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Planaria deploy an ancient gene expression program in the course of organ regeneration

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Washington, Apr 9 : In a bold attempt to make body parts using stem cells, scientists in a north London hospital are growing noses, ears and blood vessels in the laboratory.

It is among several labs around the world, including in the US, that are working on the futuristic idea of growing custom-made organs in the lab, Fox News reported.

While only a handful of patients have received the British lab-made organs so far- including tear ducts, blood vessels and windpipes - researchers hope they will soon be able to transplant more types of body parts into patients, including what would be the world's first nose made partly from stem cells.

Alexander Seifalian at University College London, the scientist leading the effort said that it's like making a cake and they just use a different kind of oven.

During a recent visit to his lab, Seifalian showed off a sophisticated machine used to make molds from a polymer material for various organs.

Last year, he and his team made a nose for a British man who lost his to cancer. Scientists added a salt and sugar solution to the mold of the nose to mimic the somewhat sponge-like texture of the real thing. Stem cells were taken from the patient's fat and grown in the lab for two weeks before being used to cover the nose scaffold. Later, the nose was implanted into the man's forearm so that skin would grow to cover it.

--ANI (Posted on 10-04-2014)

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Originally posted here:
Scientists growing human body parts in lab using stem cells

Professor Alexander Seifalian poses for photographs with a synthetic polymer nose at his research facility in the Royal Free Hospital in London, Monday, March 31, 2014. In a north London hospital, scientists are growing noses, ears and blood vessels in the laboratory in a bold attempt to make body parts using stem cells. AP

In a north London hospital, scientists are growing noses, ears and blood vessels in the laboratory in a bold attempt to make body parts using stem cells.

It is among several labs around the world, including in the U.S., that are working on the futuristic idea of growing custom-made organs in the lab.

5 Photos

In a north London hospital, scientists are growing noses, ears and blood vessels in attempt to make body parts using stem cells

"It's like making a cake," said Alexander Seifalian at University College London, the scientist leading the effort. "We just use a different kind of oven."

During a recent visit to his lab, Seifalian showed off a sophisticated machine used to make molds from a polymer material for various organs.

Last year, he and his team made a nose for a British man who lost his to cancer. Scientists added a salt and sugar solution to the mold of the nose to mimic the somewhat sponge-like texture of the real thing. Stem cells were taken from the patient's fat and grown in the lab for two weeks before being used to cover the nose scaffold. Later, the nose was implanted into the man's forearm so that skin would grow to cover it.

Seifalian said he and his team are waiting for approval from regulatory authorities to transfer the nose onto the patient's face but couldn't say when that might happen

The potential applications of lab-made organs appear so promising even the city of London is getting involved: Seifalian's work is being showcased on Tuesday as Mayor Boris Johnson announces a new initiative to attract investment to Britain's health and science sectors so spin-off companies can spur commercial development of the pioneering research.

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Ears, noses grown from stem cells in lab dishes

LONDON In a north London hospital, scientists are growing noses, ears and blood vessels in the laboratory in a bold attempt to make body parts using stem cells.

It is among several labs around the world, including in the US, that are working on the futuristic idea of growing custom-made organs in the lab.

While only a handful of patients have received the British lab-made organs so far including tear ducts, blood vessels and windpipes researchers hope they will soon be able to transplant more types of body parts into patients, including what would be the worlds first nose made partly from stem cells.

Its like making a cake, said Alexander Seifalian at University College London, the scientist leading the effort. We just use a different kind of oven.

Dr. Michelle Griffin, a plastic surgery research fellow, holds a synthetic polymer ear.Photo: AP

During a recent visit to his lab, Seifalian showed off a sophisticated machine used to make molds from a polymer material for various organs.

Last year, he and his team made a nose for a British man who lost his to cancer. Scientists added a salt and sugar solution to the mold of the nose to mimic the somewhat sponge-like texture of the real thing. Stem cells were taken from the patients fat and grown in the lab for two weeks before being used to cover the nose scaffold. Later, the nose was implanted into the mans forearm so that skin would grow to cover it.

Seifalian said he and his team are waiting for approval from regulatory authorities to transfer the nose onto the patients face but couldnt say when that might happen.

The potential applications of lab-made organs appear so promising, even the city of London is getting involved: Seifalians work is being showcased on Tuesday as Mayor Boris Johnson announces a new initiative to attract investment to Britains health and science sectors so spin-off companies can spur commercial development of the pioneering research.

The polymer material Seifalian uses for his organ scaffolds has been patented and hes also applied for patents for their blood vessels, tear ducts and windpipe. He and his team are creating other organs including coronary arteries and ears. Later this year, a trial is scheduled to start in India and London to test lab-made ears for people born without them.

Original post:
Sci-fi meets reality as stem cells are turned into noses, ears