The parents of a 2-year-old Pasadena girl who was diagnosed with an aggressive form of leukemia were this week renewing calls for help in their search for a bone marrow donor after stem cells donated from the girls father failed to help.

Sofia Flores, shown in a family photo, needs a bone marrow donor.

Sofia Flores story first came to light in October 2013 when her parents asked for help in finding a bone marrow donor for their daughter.

Sofia needed a marrow transplant to combat acute myeloid leukemia, according to A3M, a Los Angeles nonprofit that is helping Sofias parents seek a match for the little girl.

However, after an extensive search, no match was found.

On Jan. 23, her father donated his stem cells to her, which was the only alternative available at the time, according to Erica Westfall, Sofias mother.

But the treatment was not successful and Sofias cancer relapsed.

Sofias last chance for survival would be a transplant from an unrelated donor in the next two months, according to her mother.

Weve been searching for a bone marrow match even harder because this is her last chance, her father Ignacio Flores said in a video released to news media on Monday.

Sofia has not found a donor through the Be the Match registry, in part because her mixed-race ethnicity makes it difficult to find a compatible donor, according to A3M. Sofia is half white and half Mexican.

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Father of 2-Year-Old in Need of Bone Marrow: This Is Her Last Chance