Delray Beach, FL (PRWEB) March 27, 2015

Inspired by the latest advances in skin aging, BABORs Research and Innovation Center has developed a groundbreaking new skincare innovation: the anti-aging collection ReVersive, with the ultra-effective RE-YOUTH COMPLEX.

ReVersive is unique, as it contains a high-performance formula with four active ingredients that interact in perfect synergy. Designed as a complete anti-aging system, ReVersive restores youthful radiance and luminosity, leaving the complexion looking firmer and smoother with a beautifully even appearance.

VISIBLE EFFECTS FOR TIMELESSLY BEAUTIFUL SKIN

In a recent study conducted by the independent research organization, Derma Consult, the ReVersive collection showed impressive results. Testing was conducted on 100 women, aged 35 to 67, and in just 4 weeks time users reported the following exciting results:

99% MORE YOUTHFUL APPEARANCE 87% ENHANCED RADIANCE 90% FIRMER SKIN

THE RE-YOUTH COMPLEX

Telovitin: Keeps cells younger for longer Telovitin, an active ingredient based on Nobel Prize-winning research, combats skin aging at its source: cell activity. It protects the telomeres (the ends of the chromosomes) and thus extends the life cycle of the skin cells.

Agicyl: Activates defenses against skin aging This multifunctional active ingredient, which is extracted from the stem cells of the Alpine plant Globularia cordifolia, prevents the break down of the collagen fibers so that the skin retains its elasticity. It also neutralizes free radicals and environmental aggressors.

Lumicol: Creates luminosity and radiance The active radiance-boosting ingredient Lumicol, which is extracted from microalgae, can activate a protein that destroys these dark pigmentation and age spots to ensure an even-looking complexion and restore radiance.

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BABOR Launches Innovative Anti-Aging Collection ReVersive

EVEN without surgery, one can now experience a dramatic improvement and even cure on health concerns such as diabetes, cancer, HIV, and cardiovascular diseases. This is through the stem cell technology and telomere science.

Dr. Marc Lavaro Jr., an expert on general & ocular oncology, general & ocular pharmacology, pediatric ophthalmic medicine, and Science of Epigenetics said these new technology are considered as breakthrough which repairs and rejuvenates the cells.

Lavaro, head of a molecular biology research in Gifu Prefecture, Japan and Osato Research Institute, Tokyo Japan stressed that stem cell is a kind of cell that can duplicate all kinds of cell which is why it can repair a damaged heart for instance.

In his book entitled 278+ Growth Factors which he is set to publish, he also mentioned that there are also certain organs which do not regenerate like the heart and brain but through stem cells it can revitalize.

Growth factors are stem cell stimulators that address medical conditions including diseases. Each growth factor is equivalent to 1 disease. For example, in a tumor kidney problem, stem cells produce growth factors to combat it.

Another technology is the telomere science under science of Epigenetics. Telomere is part of the chromosome and it protects it. It is responsible for the cell division and daily produces new cell to replace the dead cells.

Ang cell natin is designed to last forever but and pag-ikli ng telomere ang cause of sickness. Pero pwede na siyang marepair. Activator enzyme siya kaya reverse telomere rejuvenate cell, Lavaro explained.

The good news is the stem technology is now in the market and it comes in the form of liquid gel, capsule, and syrup. This is produced by Jeunesse , an exclusive patent pending stem cell technology advance technology, science of epigenetics, and stem cell science technology. It is also cheaper compared to the old stem technology wherein one has to pay for at least 700,000 to more than one million pesos per shot.

Jeunesse is a product of medical research conducted by Dr. Nathan Newman, the father of stem cell technology and world renowned for his cosmetic surgery and innovator of stem cell lift cutting edge cosmetic surgery, without cutting.

Dapat conscious tayo sa health natin at alamin ang tinatake natin if nagwowork talaga o hype lamang ng company, Lavaro added.

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Sungduan: Growth factors

An injection of a patient's bone marrow stem cells during rotator cuff surgery significantly improved healing and tendon durability, according to a study presented today at the 2015 Annual Meeting of the American Academy of Orthopaedic Surgeons (AAOS).

Each year in the U.S., more than 2 million people have rotator cuff surgery to re-attach their shoulder tendon to the head of the humerus (upper arm bone). Rotator cuff tears can occur during a fall or when lifting an extremely heavy object; however, most tears are the result of aging and overuse.

The French study, of which a portion appeared in the September 2014 issue of International Orthopaedics, included 90 patients who underwent rotator cuff surgery. Researchers tried to make the two groups as equivalent as possible based on rotator cuff tear size, tendon rupture location, dominate shoulder, gender and age. Forty-five of the patients received injections of bone marrow concentrate (BMC) mesenchymal stem cells (MSCs) at the surgical site, and 45 had their rotator cuff repaired or reattached without MSCs.

Patient ultrasound images were obtained each month following surgery for 24 months. In addition, MRI images were obtained of patient shoulders at three and six months following surgery, and at one year, two years, and 10 years following surgery.

At six months, all 45 of the patients who received MSCs had healed rotator cuff tendons, compared to 30 (67 percent) of the patients who did not receive MSCs. The use of bone marrow concentrate also prevented further ruptures or retears. At 10 years after surgery, intact rotator cuffs were found in 39 (87 percent) of the MSC patients, but just 20 (44 percent) of the non-MSC patients.

In addition, "some retears or new tears occurred after one year," said Philippe Hernigou, MD, an orthopaedic surgeon at the University of Paris and lead study author. "These retears were more frequently associated with the control group patients who were not treated with MSCs.

"While the risk of a retear after arthroscopic repair of the rotator cuff has been well documented, publications with long-term follow-up (more than three years) are relatively limited," said Dr. Hernigou. "Many patients undergoing rotator cuff repair surgery show advanced degeneration of the tendons, which are thinner and atrophic (more likely to degenerate), probably explaining why negative results are so often reported in the literature, with frequent post-operative complications, especially retear. Observations in the MSC treatment group support the potential that MSC treatment has both a short-term and long-term benefit in reducing the rate of tendon retear."

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The above story is based on materials provided by American Academy of Orthopaedic Surgeons. Note: Materials may be edited for content and length.

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Stem cells may improve tendon healing, reduce retear risk in rotator cuff surgery

Tampa, FL. (PRWEB) March 26, 2015

This month, the Lung Institute has started treating people suffering from chronic lung diseases with stem cells extracted from their bone marrow. This treatment protocol is added to the two other treatment options offered by the Lung Institute: venous (blood-derived) and adipose (fat-derived) stem cell therapy.

The bone marrow and adipose treatments offer the highest concentration of stem cells and allow for the cells to be reintroduced directly into the lungs through a nebulizer. Given this added benefit, most patients in the past opted to receive the adipose treatment over venous. However, many patients have other medical conditions that preclude them from choosing the adipose treatment. Since the number of stem cells harvested from a bone marrow procedure matches that of the adipose procedure, patients that have previously only qualified for the venous procedure are now eligible for a treatment option that produces the highest chance of success.

Patients are often surprised by the simplicity of these minimally invasive procedures, but with cutting-edge technology and the patient-centric clinical team at the Lung Institute, patients can rest assured that they are in good hands. Throughout the entire treatment process, patients have the opportunity to get any questions immediately answered by our knowledgeable medical staff. The Lung Institute clinical team remains in contact with patients after treatment and works together with the patients physician and pulmonologist to create a strong support system for the patient.

About the Lung Institute At the Lung Institute, we are changing the lives of hundreds of people across the nation through the innovative technology of regenerative medicine. We are committed to providing patients a more effective way to address pulmonary conditions and improve their quality of life. Our physicians, through their designated practices, have gained worldwide recognition for the successful application of revolutionary minimally invasive stem cell therapies. With over a century of combined medical experience, our doctors have established a patient experience designed with the highest concern for patient safety and quality of care. For more information, visit our website at LungInstitute.com, like us on Facebook, follow us on Twitter or call us today at (855) 313-1149.

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Lung Institute Announces New Treatment with Bone Marrow

LAS VEGAS, March 26, 2015 /PRNewswire-USNewswire/ -- An injection of a patient's bone marrow stem cells during rotator cuff surgery significantly improved healing and tendon durability, according to a study presented today at the 2015 Annual Meeting of the American Academy of Orthopaedic Surgeons (AAOS).

Each year in the U.S., more than 2 million people have rotator cuff surgery to re-attach their shoulder tendon to the head of the humerus (upper arm bone). Rotator cuff tears can occur during a fall or when lifting an extremely heavy object; however, most tears are the result of aging and overuse.

The French study, of which a portion appeared in the September 2014 issue of International Orthopaedics, included 90 patients who underwent rotator cuff surgery. Researchers tried to make the two groups as equivalent as possible based on rotator cuff tear size, tendon rupture location, dominate shoulder, gender and age. Forty-five of the patients received injections of bone marrow concentrate (BMC) mesenchymal stem cells (MSCs) at the surgical site, and 45 had their rotator cuff repaired or reattached without MSCs.

Patient ultrasound images were obtained each month following surgery for 24 months. In addition, MRI images were obtained of patient shoulders at three and six months following surgery, and at one year, two years, and 10 years following surgery.

At six months, all 45 of the patients who received MSCs had healed rotator cuff tendons, compared to 30 (67 percent) of the patients who did not receive MSCs. The use of bone marrow concentrate also prevented further ruptures or retears. At 10 years after surgery, intact rotator cuffs were found in 39 (87 percent) of the MSC patients, but just 20 (44 percent) of the non-MSC patients.

In addition, "some retears or new tears occurred after one year," said Philippe Hernigou, MD, an orthopaedic surgeon at the University of Paris and lead study author. "These retears were more frequently associated with the control group patients who were not treated with MSCs.

"While the risk of a retear after arthroscopic repair of the rotator cuff has been well documented, publications with long-term follow-up (more than three years) are relatively limited," said Dr. Hernigou. "Many patients undergoing rotator cuff repair surgery show advanced degeneration of the tendons, which are thinner and atrophic (more likely to degenerate), probably explaining why negative results are so often reported in the literature, with frequent post-operative complications, especially retear. Observations in the MSC treatment group support the potential that MSC treatment has both a short-term and long-term benefit in reducing the rate of tendon retear."

Study abstract

View 2015 AAOS Annual Meeting disclosure statements

About AAOS

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Stem cells may significantly improve tendon healing, reduce retear risk in rotator cuff surgery

Beneath several blankets and a stuffed giraffe in her UC San Francisco hospital bed, 11-year-old Myla Cunanan is resting after a morning of dialysis to treat a kidney-related complication from her bone marrow transplant last year.Myla is sedated and tired, but not enough to silence her spritely personality.Mom, you put the cover on backwards! she exclaimed, disassembling her iPhone from its case and flipping it around as her mother, Leyna Cunanan, laughed and lovingly stroked the hand of her youngest daughter.She makes us all brave around her, Leyna said of her daughter. She knows that there is a purpose for her for being here. Tuesday marked one year to the day since Myla was diagnosed with myeloid sarcoma, a rare cancer in which a solid collection of leukemic cells occur outside of the bone marrow. The last year also thrust Myla into the spotlight as she and her family sought to find a bone marrow donor, a mission that turned out to be impossible due to a severe lack of Asian donors worldwide.Myla is Filipino-American, and when doctors told her after three rounds of chemotherapy in spring 2014 that she urgently needed a bone marrow transplant, her family learned just how difficult it is to find a match.In fact, Asians comprise just 6 percent of donors with Be The Match Registry, the largest and most diverse marrow registry in the world.The rarer your ethnic subtype is, at least in the U.S., the less likely we are to find you a good donor, said Dr. Christopher Dvorack, who has treated Myla since last year and is an assistant professor of clinical pediatrics in the Division of Allergy, Immunology, and Blood and Marrow Transplant at UC San Francisco.Last summer, her family registered about 300 donors through drives at their church, Mylas school and local shopping centers, and shared Mylas plight on social media with a photo of Myla holding a sign that reads, Will you marrow me?But a match was not found, and by August, doctors told Mylas family they would need to use a half-match donor, which was Mylas father.There are two main ways to donate bone marrow. The first is to have needles inserted into hip bones to extract a small amount of bone marrow. The second requires four days of injections of medicine designed to stimulate bone marrow and cause it to release stem cells from the bone marrow into the blood.The problem with half-match donors is the patients immune system can reject the donated bone marrow, which is what happened to Myla, Dvorack explained.She initially did well, she then later developed a complication that has kept her in the hospital, he said.The complication, thrombotic microangiopathy with renal involvement, means Mylas kidneys function less than 15 percent. She was subsequently diagnosed as chronic kidney disease Stage 5, and has been receiving hemodialysis several times a week.But her family remain advocates for the need for more bone marrow donors, particularly among ethnic minorities.We didnt find a match for Myla ... but we would like to continue to [hold] drives for other patients, her mother said.Ruby Law, a recruitment director for the Asian American Donor Program based in Alameda, worked with Myla and her family last year to seek a donor and said their efforts have extended beyond simply finding a match.Mylas family is very passionate about raising awareness of marrow and blood stem cell donation, Law said.Since Mylas most recent hospitalization, which began a week before Thanksgiving, her mother has lived with her at UCSF. Myla was among the 126 patients transferred from the UCSF Parnassus Campus to the new complex at Mission Bay on Feb. 1.Recently, Myla has been writing a book to help other kids going through similar journeys.When you read this book, I want you to think positive always, the last line of the opening letter states.And that pretty much sums up Mylas attitude, according to her doctor and family. Despite having been hospitalized for the majority of the past year, Myla insists she has plenty to be thankful for. While hospitalized, Myla has held book, bracelet and band-aid drives.There are days, of course, when she doesnt feel good, her mother said. But she always thinks about other people. She likes to give.To sign up as a donor, visit http://www.aadp.org or call (510) 568-3700.

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One year after cancer diagnosis, Bay Area girl continues to highlight need for Asian bone marrow donors

My bone marrow began sending me messages long before I Learned to listen..I was exhausted, pale, drained, and tired all of the time. I eventually landed myself in the emergency room, and was handed a full membership to the cancer club. I was diagnosed with multiple myeloma, an incurable blood cancer.

My bone marrow began sending me messages long before I Learned to listen..I was exhausted, pale, drained, and tired all of the time. I eventually landed myself in the emergency room, and was handed a full membership to the cancer club. I was diagnosed with multiple myeloma, an incurable blood cancer.

That was June 23, 2009.

As it turned out I was very fortunate. I beat the statistical odds and circumstances were in my favour. After four months of chemo and steroids, I was able to use my own stem cells in what is called an autologous stell cell transplant.

My stem cell transplant was a journey to my very core. It's like witnessing a rebirth. It's awe-inspiring and essential. Visualizing those 'yellow' cells stream their way back into my bone marrow opened my eyes to the singular power stem cells bring into our world.

But I was also reminded of Michael Pinto the undertaker in Bombay.

'Grave Problems Resurrected here'

That's so not gonna happen. Not on my resurrection.

My passage through illness taught me that the knowledge of the curative properties of stem cells needs to be shared to offer hope of renewed life. If you knew what medical science can do with stem cells, and if you saw what I did in the labs, through microscropes, you too would feel like using both hands to scoop those secrets out into the world.

Occasionally I gloss over my past cancer club membership--my treatment, my illnessbut then I am remember what a profound reboot my body has gone through, and I remember why. It's true that the deepest crises are moments of great opportunity; an event that shocks you into seeing with your heart. It is a place that combines survival with celebration.

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My message in a bottle, writes Lisa Ray

A federal public servant has lost a legal bid to have taxpayers pay for experimental stem cell treatment on his dodgy knees.

The Administrative Appeals Tribunal has knocked back an appeal by Customs officer Vic Kaplicas to force insurer Comcare to pay $13,400 for the new treatment, instead saying he could have a tried-and-tested double knee replacement.

But the 49-year-old border official says he worries he cannot pass his department's fitness tests if he undergoes the knee replacements, which will leave him unable to run.

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The former triathlete, who had to give up his sport because of his bad knees, said he was keen to avoid the "radical but effective" replacements for as long as possible.

Mr Kaplicas hurt his left knee working at Sydney's Mascot Airport in 2000, then injured his right knee 10 years later at Kingsford-Smith.

He managed the pain in his knees, which have since developed osteoarthritis, for years using over-the-counter painkillers, physio, exercises and injections but Mr Kaplicas' doctors say a more permanent solution is now needed.

In June 2012, Sydney knee specialist Sam Sorrenti asked Comcare to pay for bilateral knee stem cell assisted arthroscopic surgery for Mr Kaplicas.

The cost of the procedure was estimated at $13,464.00 for arthroscopy, stem cell harvesting and injection, and a "HiQCell procedure".

Dr Sorrenti said the knee replacements were not a good idea for a man of Mr Kaplicas' age, arguing the new knees would last 15 years at best, were intended for older people who are less concerned with physical activity, and left no further options.

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No stem cell treatment for public servant's dodgy knee

Irvine, Calif., March 26, 2015 -- Leslie Thompson of the Sue & Bill Gross Stem Cell Research Center at UC Irvine has been awarded $5 million by the California Institute for Regenerative Medicine to continue her CIRM-funded effort to develop stem cell treatments for Huntington's disease.

The grant supports her next step: identifying and testing stem cell-based treatments for HD, an inherited, incurable and fatal neurodegenerative disorder. In this project, Thompson and her colleagues will establish an HD therapy employing human embryonic stem cells that can be evaluated in clinical trials.

Over the past seven years, Thompson, a UCI professor of psychiatry & human behavior and neurobiology & behavior, and her team have used CIRM funding to produce stem cell lines "reprogrammed" from the skin cells of individuals carrying the Huntington's genetic mutation in order to study the disease. In addition, they conducted basic and early-stage transitional studies to develop a stem cell-based technique to treat areas of the brain susceptible to HD.

"These stem cells offer a possible long-term treatment approach that could relieve the tremendous suffering experienced by HD patients and their families," said Thompson, who's also affiliated with UCI's Institute for Memory Impairments and Neurological Disorders (UCI MIND). "We appreciate CIRM and the millions of people in the state of California for generously supporting breakthrough stem cell research."

With this award, CIRM has granted Thompson $10.3 million for her HD work. Overall, UCI has received $105 million from the state-funded agency.

Thompson said that her group has identified a highly promising neural stem cell line that shows disease-modifying activity in HD mice. These neural stem cells were grown from human embryonic stem cells at UC Davis. The researchers also will conduct essential preclinical efficacy and safety studies in HD mice with these cells.

Over the span of the 2-year grant, Thompson said, the goal is to finalize work that will lead to a pre-investigational-new-drug meeting with the Food & Drug Administration and a path forward for clinical trials with the neural stem cells.

"This investment will let us further test the early promise shown by these projects," said Jonathan Thomas, chair of the CIRM governing board. "Preclinical work is vital in examining the feasibility, potential effectiveness and safety of a therapy before we try it on people. These projects all showed compelling evidence that they could be tremendously beneficial to patients. We want to help them build on that earlier research and move the projects to the next level."

HD is a devastating degenerative brain disorder with no disease-modifying treatment or cure. Current approaches only address certain symptoms of HD and do not change its course.

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UCI team gets $5 million to create stem cell treatment for Huntington's disease

Stem Cell Therapy For Multiple Sclerosis
Back for round 2. After treatment in 2014 Beverly had improvement in her energy level, balance, walking and had colors come into her vision for the first time in 10 years. Beverly went blind...

By: Stem Cell Patient

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Stem Cell Therapy For Multiple Sclerosis - Video

Bobby Chia (Foto by Allan Defensor)

THERE is a lot of hype going on in some circles about stem cell therapy: Is it a cure-all? Is it the elusive fountain of youth?

Wikipedia definesstem cell therapy as the use of stem cells to treat or prevent a disease or condition.The process involves the administration of live whole cells or maturation of a specific cell population in a patient for the treatment of the disease as has been done in bone marrow transplants.

Bobby Chia, a Thai national who was in Cebu for a brief visit, said that stem cell therapy has been done in Villa Medica, Germany, since the 1960s. He learned about it 10 years ago when his mother had cancer and he looked around for the best medical care for her and found it in Villa Medica. It made her so much better (she can even play tennis now) that four years ago, Chia bought the clinic being run by Dr. Geoffrey Huertgen, a third generation doctor of that clinic.

The stem cell can be taken from the patient himself, but Chia says this stem source is naturally as old as the patient himself. Villa Medica chooses to use stem cells from fetuses of sheep (he said that stem cells from any mammal would be the same, but sheep stem cells are the ones more readily available). The process for Villa Medica, says Chia, involves the designing of a cocktail of stem cells to address whatever needs correction. If the eyes are not good, we choose the eyes. If the ears are not good, we choose the ears. If the heart is not good, we choose the heart. The procedure has been known to treat diseases and ailments like Parkinsons, diabetes, hypertension, migraine, allergies etc.

It (stem cell therapy) does not make you 18 years old all over again but it energizes you; from not being able to walk to walking again. It gives you a better quality of life. It is not a quick cure, but for a lot of people it is. We offer a choice for people who have no hope, Chia shares. But my main target is people who are not sick, Chia states, referring to athletes, models, professionals who want to have a better quality of life.

Chia says there are four doctors in Villa Medica. Since the cell therapy is patient-specific, the patient needs to stay four days and four nights at the clinic: for a detox program, for physical check-up, for interview, for determining the cocktail of stem cells to be used and how. The result, Chia says,is not immediate. It may take six weeks or even longer for the stem cells to do their work.

Chia says he has had about 150 patients coming from Cebu. Leaf through the pages of the local papers, one of them might just be there!

Published in the Sun.Star Cebu newspaper on March 27, 2015.

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On stem cell therapy, benefits

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Newswise (New York March 25, 2015) Induced pluripotent stem cells (iPSCs)adult cells reprogrammed back to an embryonic stem cell-like statemay better model the genetic contributions to each patient's particular disease. In a process called cellular reprogramming, researchers at Icahn School of Medicine at Mount Sinai have taken mature blood cells from patients with myelodysplastic syndrome (MDS) and reprogrammed them back into iPSCs to study the genetic origins of this rare blood cancer. The results appear in an upcoming issue of Nature Biotechnology.

In MDS, genetic mutations in the bone marrow stem cell cause the number and quality of blood-forming cells to decline irreversibly, further impairing blood production. Patients with MDS can develop severe anemia and in some cases leukemia also known as AML. But which genetic mutations are the critical ones causing this disease?

In this study, researchers took cells from patients with blood cancer MDS and turned them into stem cells to study the deletions of human chromosome 7 often associated with this disease.

With this approach, we were able to pinpoint a region on chromosome 7 that is critical and were able to identify candidate genes residing there that may cause this disease, said lead researcher Eirini Papapetrou, MD, PhD, Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai.

Chromosomal deletions are difficult to study with existing tools because they contain a large number of genes, making it hard to pinpoint the critical ones causing cancer. Chromosome 7 deletion is a characteristic cellular abnormality in MDS and is well-recognized for decades as a marker of unfavorable prognosis. However, the role of this deletion in the development of the disease remained unclear going into this study.

Understanding the role of specific chromosomal deletions in cancers requires determining if a deletion has observable consequences as well as identifying which specific genetic elements are critically lost. Researchers used cellular reprogramming and genome engineering to dissect the loss of chromosome 7. The methods used in this study for engineering deletions can enable studies of the consequences of alterations in genes in human cells.

Genetic engineering of human stem cells has not been used for disease-associated genomic deletions, said Dr. Papapetrou. This work sheds new light on how blood cancer develops and also provides a new approach that can be used to study chromosomal deletions associated with a variety of human cancers, neurological and developmental diseases.

Reprogramming MDS cells could provide a powerful tool to dissect the architecture and evolution of this disease and to link the genetic make-up of MDS cells to characteristics and traits of these cells. Further dissecting the MDS stem cells at the molecular level could provide insights into the origins and development of MDS and other blood cancers. Moreover, this work could provide a platform to test and discover new treatments for these diseases.

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Mount Sinai Researchers Discover Genetic Origins of Myelodysplastic Syndrome Using Stem Cells

(New York - March 25, 2015) Induced pluripotent stem cells (iPSCs) -- adult cells reprogrammed back to an embryonic stem cell-like state--may better model the genetic contributions to each patient's particular disease. In a process called cellular reprogramming, researchers at Icahn School of Medicine at Mount Sinai have taken mature blood cells from patients with myelodysplastic syndrome (MDS) and reprogrammed them back into iPSCs to study the genetic origins of this rare blood cancer. The results appear in an upcoming issue of Nature Biotechnology.

In MDS, genetic mutations in the bone marrow stem cell cause the number and quality of blood-forming cells to decline irreversibly, further impairing blood production. Patients with MDS can develop severe anemia and in some cases leukemia also known as AML. But which genetic mutations are the critical ones causing this disease?

In this study, researchers took cells from patients with blood cancer MDS and turned them into stem cells to study the deletions of human chromosome 7 often associated with this disease.

"With this approach, we were able to pinpoint a region on chromosome 7 that is critical and were able to identify candidate genes residing there that may cause this disease," said lead researcher Eirini Papapetrou, MD, PhD, Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai.

Chromosomal deletions are difficult to study with existing tools because they contain a large number of genes, making it hard to pinpoint the critical ones causing cancer. Chromosome 7 deletion is a characteristic cellular abnormality in MDS and is well-recognized for decades as a marker of unfavorable prognosis. However, the role of this deletion in the development of the disease remained unclear going into this study.

Understanding the role of specific chromosomal deletions in cancers requires determining if a deletion has observable consequences as well as identifying which specific genetic elements are critically lost. Researchers used cellular reprogramming and genome engineering to dissect the loss of chromosome 7. The methods used in this study for engineering deletions can enable studies of the consequences of alterations in genes in human cells.

"Genetic engineering of human stem cells has not been used for disease-associated genomic deletions," said Dr. Papapetrou. "This work sheds new light on how blood cancer develops and also provides a new approach that can be used to study chromosomal deletions associated with a variety of human cancers, neurological and developmental diseases."

Reprogramming MDS cells could provide a powerful tool to dissect the architecture and evolution of this disease and to link the genetic make-up of MDS cells to characteristics and traits of these cells. Further dissecting the MDS stem cells at the molecular level could provide insights into the origins and development of MDS and other blood cancers. Moreover, this work could provide a platform to test and discover new treatments for these diseases.

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This study was supported by grants from the National Institutes of Health, the American Society of Hematology, the Sidney Kimmel Foundation for Cancer Research, the Aplastic Anemia & MDS International Foundation, the Ellison Medical Foundation, the Damon Runyon Cancer Research Foundation, the University of Washington Royalty Research Fund, and a John H. Tietze Stem Cell Scientist Award.

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Researchers discover genetic origins of myelodysplastic syndrome using stem cells

Julie Gramyk 3 21 2015 Youtube
Julie Gramyk, Medical Esthetic, explains how Momentis #39; new skincare system is the first in the world to penetrate beyond the skin #39;s barrier and target the skin #39;s stem cells resulting in rebuilding...

By: judyrstak

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Julie Gramyk 3 21 2015 Youtube - Video

5 hours ago

Scientists at the University of Copenhagen have captured thousands of progenitor cells of the pancreas on video as they made decisions to divide and expand the organ or to specialize into the endocrine cells that regulate our blood sugar levels.

The study reveals that stem cells behave as people in a society, making individual choices but with enough interactions to bring them to their end-goal. The results could eventually lead to a better control over the production of insulin-producing endocrine cells for diabetes therapy.

The research is published in the scientific journal PLOS Biology.

Why one cell matters

In a joint collaboration between the University of Copenhagen and University of Cambridge, Professor Anne Grapin- Botton and a team of researchers including Assistant Professor Yung Hae Kim from DanStem Center focused on marking the progenitor cells of the embryonic pancreas, commonly referred to as 'mothers', and their 'daughters' in different fluorescent colours and then captured them on video to analyse how they make decisions.

Prior to this work, there were methods to predict how specific types of pancreas cells would evolve as the embryo develops. However, by looking at individual cells, the scientists found that even within one group of cells presumed to be of the same type, some will divide many times to make the organ bigger while others will become specialized and will stop dividing.

The scientists witnessed interesting occurrences where the 'mother' of two 'daughters' made a decision and passed it on to the two 'daughters' who then acquired their specialization in synchrony. By observing enough cells, they were able to extract logic rules of decision-making, and with the help of Pau Ru, a mathematician from the University of Cambridge, they developed a mathematical model to make long-term predictions over multiple generations of cells.

Stem cell movies

'It is the first time we have made movies of a quality that is high enough to follow thousands of individual cells in this organ, for periods of time that are long enough for us to follow the slow decision process. The task seemed daunting and technically challenging, but fascinating", says Professor Grapin-Botton.

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Stem cells make similar decisions to humans

Researchers at the University of Cambridge have grown functional "mini-lungs" using stems cells derived from the skin cells of patients with a debilitating lung disease. Not only can the development help them in coming up with effective treatments for specific lung diseases like cystic fibrosis, but the process has the potential to be scaled up to screen thousands of new compounds to identify potential new drugs.

Creating miniature organoids has been the focus of many a research group, as it allows scientists to better understand the processes that take place inside an organ, figure out how specific diseases occur and develop or even work towards creating bioengineered lungs.

The research team from the Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute studied a lung disease called cystic fibrosis, which is caused by genetic mutation and shortens a patient's average lifespan. Patients have great difficulty breathing as the lungs are overwhelmed by thickened mucus.

To create working mini-lungs, the researchers took skin cells from patients with the most common form of cystic fibrosis and reprogrammed them to an induced pluripotent state (iPS), which allows the cells to grow into a different type of cell inside the body.

They then activated a process called gastrulation which pushes the cells to form distinct layers such as the endoderm and foregut. The cells were then pushed further to form distal airway tissue, the part of the lung that deals with exchange of gases.

In a sense, what weve created are mini-lungs," says Dr Nick Hannan, the lead researcher. While they only represent the distal part of lung tissue, they are grown from human cells and so can be more reliable than using traditional animal models, such as mice."

To find out whether the mini lungs could actually be used to screen drugs, the team tested them out with the aid of chloride-sensitive fluorescent dye. Cells from cystic fibrosis patients typically malfunction and don't allow the chloride to pass through, so there's no change in fluorescence levels.

The team added a molecule that's currently undergoing clinical trials and noted a change in fluorescence, signaling that it was effective in getting the diseased lung cells to function properly and that the mini lungs could, in principle, be used to test potential new drugs.

"Were confident this process could be scaled up to enable us to screen tens of thousands of compounds and develop mini-lungs with other diseases such as lung cancer and idiopathic pulmonary fibrosis," says Dr Hannan. "This is far more practical, should provide more reliable data and is also more ethical than using large numbers of mice for such research."

The research was published in the journal Stem Cells and Development.

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Scientists create functioning "mini-lungs" to study cystic fibrosis

CRISPR-Cas9 is a powerful new tool for editing the genome. For researchers around the world, the CRISPR-Cas9 technique is an exciting innovation because it is faster and cheaper than previous methods. Now, using a molecular trick, Dr. Van Trung Chu and Professor Klaus Rajewsky of the Max Delbrck Center for Molecular Medicine (MDC) Berlin-Buch and Dr. Ralf Khn, MDC and Berlin Institute of Health (BIH), have found a solution to considerably increase the efficiency of precise genetic modifications by up to eightfold.

"What we used to do in years, we can now achieve in months," said gene researcher and immunologist Klaus Rajewsky, indicating the power of this new genome-editing technology. CRISPR-Cas9 not only speeds up research considerably - at the same time it is much more efficient, cheaper and also easier to handle than the methods used so far.

The CRISPR-Cas9 technology allows researchers to transiently introduce DNA double-strand breaks into the genome of cells or model organisms at genes of choice. In these artificially produced strand breaks, they can insert or cut out genes and change the genetic coding according to their needs.

Mammalian cells are able to repair DNA damage in their cells using two different repair mechanisms. The homology-directed repair (HDR) pathway enables the insertion of preplanned genetic modifications using engineered DNA molecules that share identical sequence regions with the targeted gene and which are recognized as a repair template. Thus, HDR repair is very precise but occurs only at low frequency in mammalian cells.

The other repair system, called non-homologous end-joining (NHEJ) is more efficient in nature but less precise, since it readily reconnects free DNA ends without repair template, thereby frequently deleting short sequences from the genome. Therefore, NHEJ repair can only be used to create short genomic deletions, but does not support precise gene modification or the insertion and replacement of gene segments.

Many researchers, including Van Trung Chu, Klaus Rajewsky and Ralf Khn, are seeking to promote the HDR repair pathway to make gene modification in the laboratory more precise in order to avoid editing errors and to increase efficiency. The MDC researchers succeeded in increasing the efficiency of the more precisely working HDR repair system by temporarily inhibiting the most dominant repair protein of NHEJ, the enzyme DNA Ligase IV. In their approach they used various inhibitors such as proteins and small molecules.

"But we also used a trick of nature and blocked Ligase IV with the proteins of adeno viruses. Thus we were able to increase the efficiency of the CRISPR-Cas9 technology up to eightfold," Ralf Khn explained. For example, they succeeded in inserting a gene into a predefined position in the genome (knock-in) in more than 60 per cent of all manipulated mouse cells. Khn has just recently joined the MDC and is head of the research group for "iPS cell based disease modeling." Before coming to the MDC, he was on the research staff of Helmholtz Zentrum Mnchen. "The expertise of Ralf Khn is very important for gene research at MDC and especially for my research group," Klaus Rajewsky said.

Concurrent with the publication of the article by the MDC researchers, Nature Biotechnology published another, related paper on CRISPR-Cas9 technology. It comes from the laboratory of Hidde Ploegh of the Whitehead Institute in Cambridge, MA, USA.

Somatic gene therapy with CRISPR-Cas9 is a goal

The new CRISPR-Cas9 technology, developed in 2012, is already used in the laboratory to correct genetic defects in mice. Researchers also plan to modify the genetic set up of induced pluripotent stem cells (iPS), which can be differentiated into specialized cell types or tissues. That is, researchers are able to use the new tool to introduce patient-derived mutations into the genome of iPS cells for studying the onset of human diseases. "Another future goal, however, is to use CRISPR-Cas9 for somatic gene therapy in humans with severe diseases," Klaus Rajewsky pointed out.

More here:
Researchers greatly increase precision of new genome editing tool

CRISPR-Cas9 is a powerful new tool for editing the genome. For researchers around the world, the CRISPR-Cas9 technique is an exciting innovation because it is faster and cheaper than previous methods. Now, using a molecular trick, Dr. Van Trung Chu and Professor Klaus Rajewsky of the Max Delbrck Center for Molecular Medicine (MDC) Berlin-Buch and Dr. Ralf Khn, MDC and Berlin Institute of Health (BIH), have found a solution to considerably increase the efficiency of precise genetic modifications by up to eightfold (Nature Biotechnology: doi:10.1038/nbt.3198)**.

"What we used to do in years, we can now achieve in months," said gene researcher and immunologist Klaus Rajewsky, indicating the power of this new genome-editing technology. CRISPR-Cas9 not only speeds up research considerably - at the same time it is much more efficient, cheaper and also easier to handle than the methods used so far.

The CRISPR-Cas9 technology allows researchers to transiently introduce DNA double-strand breaks into the genome of cells or model organisms at genes of choice. In these artificially produced strand breaks, they can insert or cut out genes and change the genetic coding according to their needs.

Mammalian cells are able to repair DNA damage in their cells using two different repair mechanisms. The homology-directed repair (HDR) pathway enables the insertion of preplanned genetic modifications using engineered DNA molecules that share identical sequence regions with the targeted gene and which are recognized as a repair template. Thus, HDR repair is very precise but occurs only at low frequency in mammalian cells.

The other repair system, called non-homologous end-joining (NHEJ) is more efficient in nature but less precise, since it readily reconnects free DNA ends without repair template, thereby frequently deleting short sequences from the genome. Therefore, NHEJ repair can only be used to create short genomic deletions, but does not support precise gene modification or the insertion and replacement of gene segments.

Many researchers, including Van Trung Chu, Klaus Rajewsky and Ralf Khn, are seeking to promote the HDR repair pathway to make gene modification in the laboratory more precise in order to avoid editing errors and to increase efficiency. The MDC researchers succeeded in increasing the efficiency of the more precisely working HDR repair system by temporarily inhibiting the most dominant repair protein of NHEJ, the enzyme DNA Ligase IV. In their approach they used various inhibitors such as proteins and small molecules.

"But we also used a trick of nature and blocked Ligase IV with the proteins of adeno viruses. Thus we were able to increase the efficiency of the CRISPR-Cas9 technology up to eightfold," Ralf Khn explained. For example, they succeeded in inserting a gene into a predefined position in the genome (knock-in) in more than 60 per cent of all manipulated mouse cells. Khn has just recently joined the MDC and is head of the research group for "iPS cell based disease modeling". Before coming to the MDC, he was on the research staff of Helmholtz Zentrum Mnchen. "The expertise of Ralf Khn is very important for gene research at MDC and especially for my research group," Klaus Rajewsky said.

Concurrent with the publication of the article by the MDC researchers, Nature Biotechnology published another, related paper on CRISPR-Cas9 technology. It comes from the laboratory of Hidde Ploegh of the Whitehead Institute in Cambridge, MA, USA.

Somatic gene therapy with CRISPR-Cas9 is a goal

The new CRISPR-Cas9 technology, developed in 2012, is already used in the laboratory to correct genetic defects in mice. Researchers also plan to modify the genetic set up of induced pluripotent stem cells (iPS), which can be differentiated into specialized cell types or tissues. That is, researchers are able to use the new tool to introduce patient-derived mutations into the genome of iPS cells for studying the onset of human diseases. "Another future goal, however, is to use CRISPR-Cas9 for somatic gene therapy in humans with severe diseases," Klaus Rajewsky pointed out.

Read this article:
MDC researchers greatly increase precision of new genome editing tool

Celprogen Released Stem Cell Active Ingredients for the Cosmetic Industry Tested and Validated in Cosmetic Products for a Decade

The present invention relates to culturing stem cells in animal free conditions has been developed and optimized by Celprogen utilizing single use bioreactor technology. The cosmetic industry has benefited from this technology for their regenerative skin care product lines. The topical application of these skin care products utilizing Celprogens Stem Cell Derived Conditioned Media have been in the market for 10 plus years.

About Celprogen Inc. Celprogen Inc. is a global Stem Cell Research & Therapeutics company which is developing a proprietary portfolio of unique therapeutics products and life science research tools that includes genetic engineering technologies, stem cell technologies for regenerative medicine, as well as bio-engineering products for tissue & organ transplants. Headquartered in Torrance, California, Celprogen is committed to the research, development, and manufacture of quality Stem Cell, Cancer Stem Cell and Primary Cell Culture products to serve our global community. Additional information about Celprogen is available at http://www.celprogen.com.

For additional information on the product line contact: Jay Sharma Phone: 310 542 8822 info@celprogen.com http://www.celprogen.com

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Celprogen Released Stem Cell Active Ingredients for the Cosmetic Industry Tested and Validated in Cosmetic Products ...

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Newswise March 24, 2015, NATIONAL HARBOR, Md. - Mesenchymal stem cell (MSC) transplantation reduced opioid tolerance and opioid-induced hyperalgesia caused by daily morphine injections in rats, according to new research. The results could herald stem cell transplantation as an innovative, safe, efficacious and cost-effective therapy to treat pain and opioid tolerance, said researchers, who presented results in a Plenary Research Highlight session at the 31st Annual Meeting of the American Academy of Pain Medicine.

Not only was opioid tolerance prevented when the rats were transplanted with MSC before repeated morphine injections, but tolerance was reversed when the rats were treated after opioid tolerance had developed, results demonstrated.

MSCs have a remarkable anti-inflammatory effect and a powerful anti-tolerance effect, said the studys principal investigator, Jianguo Cheng, M.D., Ph.D., who led the research team from the Cleveland Clinic, in Ohio. Although clinical trials are still three to five years away, he said, eventually, The results may apply to millions of patients with a wide range of pain states, including cancer pain and other intractable chronic pain that requires long-term opioid therapy.

Furthermore, Cheng characterized the procedure as practical, in light of readily available sources of stem cells, reliable stem cell technology, the simplicity of transplantation procedures and the fact that clinical trials are already underway involving autoimmune and other diseases.

The Institute of Medicine report on pain in America documented millions who suffer with chronic pain (Relieving Pain in America: A Blueprint for Transforming Prevention, Care, Education, and Research. National Academies Press [US]; 2011). Opioid therapy is a cornerstone component of pain management for many people with severe, ongoing pain; however, side effects such as tolerance and the risks posed by abuse, addiction and drug overdose limit its utility. Tolerance, a physiologic process in which the patients body adjusts to a dose and no longer achieves pain relief, is a common limitation with opioid therapy. The higher doses that result can limit effectiveness and compromise safety.

Glial cells are of growing interest in pain research and have been implicated in the development of tolerance. Glial cell activity also produces pain through the release of products that excite the nervous system, playing an important role in the spinal cord during nerve injury. Furthermore, the opioids used to treat pain, also can induce glial activity, causing pain relief to drop and unwanted opioid effects, including tolerance, dependence, reward and decreased breathing, to grow. A focus of research, then, is to separate the desired effect of pain relief from the unwanted opioid effects (Watkins et al, Trends in Pharmacological Sciences 2009;30(11): 581-91).

Interest in transplant of stem cells is another maturing research avenue (Hsu et al, Cell Transplant 2007;16(2):133-50). MSCs can differentiate into a variety of cell types and have been investigated for potential repair of damaged neural cells and for calming inflammation in the immune system to promote recovery after traumatic brain injury (Zhang et al, J Neuroinflammation 2013;10(1):106).

Following this line of research, the study investigators wondered whether they could create an anti-tolerance therapy by transplanting MSCs into the intrathecal space surrounding the spinal cord. With approval by the Cleveland Clinic Institutional Animal Care and Use Committee and funding through the Department of Defenses Congressionally Directed Medical Research Programs, they compared the withdrawal thresholds of the hind paws in response to painful mechanical and thermal stimuli in two groups of rats that received daily morphine injections. The first group was treated with MSC transplantation and the control group with phosphate-buffered saline (PBS).

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Cleveland Clinic Researchers First to Demonstrate Significant Blocking of Opioid Tolerance With Mesenchymal Stem Cell ...